Motion in Alcohol Dehydrogenase (ADH) [adh]

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Classification Known Domain Motion, Shear Mechanism [D-s-2]

Structures
6ADH Conformation 2 [ PartsList ]
8ADH Conformation 1 [ PartsList ]

Description
Shear motion with XBAaba layering: ADH enzyme has 3 moving layers in one domain that shift relative to 3 rigid layers in the other domain. This distinctive layering pattern is of the form XBAabx, where abx are the 3 moving layers and XBA the rigid layers. The interface between the two middle layers is where the major shear motion occurs. One layer of helices from the mobile domain (a) slides over a layer of helices from the motionless domain (A). Helices in these two layers, which in a sense form gears upon which the domains slide, are often crossed. Near to the a and A layer helices, the ligand binds in the interdomain cleft . Packed onto either side of the central layers of helices (a and A) are sheets (b and B) from the mobile and motionless domains, respectively. The mobile sheet (b) forms a second moving layer, which slides over the helices, and packed onto the other face of this sheet is a third layer (x) which moves with the sheet. Symmetrical to this third moving layer (x), a third motionless layer (X) is packed onto one side of the static sheet (B). In ADH layer x is made up of helices and a sheet, and layer X is made up of helices. In addition to its shear motion, ADH also has two well defined hinge points.

Particular values describing motion
Experimental Methods = xm (Traditional x-ray and Molecular Dynamics)
Creation Date = 19970822
Modification Date = 19970822

References
F Colonna-Cesari, D Perahia, M Karplus, H Eklund, C I Branden and O Tapia (1986). Interdomain motion in liver alcohol dehydrogenase: structural and energetic analysis of the hinge bending mode. J. Biol. Chem. 261: 15273-15280. [Medline info for 9615437]
H Eklund, J P Samaha, L Wallen, C I Branden, Angstroms Angstromskeson and T A Jones (1981). Structure of a triclinic ternary complex of horse liver alcohol dehydrogenase at 2.9 A resolution. J. Mol. Biol. 146: 561-587. [Medline info for 9615437]

GO terms associated with structures
Molecular functionzinc ion binding, oxidoreductase activity

Morphs

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Best representative
Morph Morph name Structure #1 Structure #2 Residues
8adh [ ] 6adh [ ] 374

User-submitted morphs
Morph Morph name Structure #1 Structure #2 Residues
Via_align 8adh [ A ] 5adh [ A ] 374

Automatic morphs
Morph Morph name Structure #1 Structure #2 Residues
Alcohol Dehydrogenase 1axg [ B ] 5adh [ A ] 374
Alcohol Dehydrogenase (E.C. 1. 1adg [ A ] 1axg [ B ] 374

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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu