Motion in Recoverin [recvin]

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Classification Known Domain Motion, Hinge Mechanism [D-h-2]

Structures 1IKU Ca-freeConformation 2 [ PartsList ] 1JSA Ca-boundConformation 3 [ PartsList ] 1REC Conformation 1 [ PartsList ]

Description Myristoylated and Unmyristoylated-CA-recoverin structures differ significantly from one another, providing insight into the functioning the mystristoyl-CA switch. Making the assumption that the x-ray structure of one CA2+-unmyristoylated recoverin was a good approximation to the R form the myristoylated protein, Ames et al. compared the two proteins. It is found that the carboxy-terminal domains of the two structures are quite similar, while their amino-terminal domains are very different (2.2+/-0.1 A rmsd versus 6.9+/- 0.1 A). The two helices of EF-1 are nearly antiparallel in the CA2+-free protein, contrasting with their nearly perpendicular orientation (108 deg.) in the CA2+-bound protein. Mechanism seems to involve a backbond hinge rotation at the domain linker. [June 1998 Update: The structure of Ca-bound myristoylated protein is indeed very much like that of the unmyristoylated Ca-bound protein, as anticipated (they are both in the R state). Per correspondance, M. Erickson]

Particular values describing motion Creation Date = 19971130 Experimental Methods = x (Traditional x-ray) Modification Date = 1998-06-24 11:08:36.000

References Ames JB, Ishima R, Tanaka T, Gordon JI, Stryer L, Ikura M (1997). Molecular mechanics of calcium-myristoyl switches. Molecular mechanics of calcium-myristoyl switches. Nature 389:198-202. [Medline info for 97441062] Ames JB, Tanaka T, Ikura M, Stryer L (1995). Nuclear magnetic resonance evidence for Ca(2+)-induced extrusion of the myristoyl group of recoverin. J Biol Chem 270(52):30909-30913. [Medline info for 96125064] Erickson, M.A., Lagnado, L., Zozulya, S., Neubert, T.A., Stryer, L., and Baylor, D.A., 1998. The effect of recombinant recoverin on the photoresponse of truncated rod photoreceptors. Proc. Natl. Acad. Sci. USA 95:6474-6479. [Medline info for 98263382] J B Ames, T Tanaka, L Stryer, and M Ikura (1996). Portrait of a Myristoyl Switch Protein. Curr. Opin. Struct. Biol. 6:432-438. [Medline info for 96391937] Tanaka, T., Ames, J.B., Harvey, T.S., Stryer, L., and Ikura, M., 1995. Sequestration of the membrane-targeting myristoyl group of recoverin in the calcium-free state. Nature 376:444-447. [Medline info for 95356838]

Data and Graphics movie (Auto Generated)

GO terms associated with structures Molecular function calcium ion binding

Morphs

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Best representative Morph Morph name Structure #1 Structure #2 Residues [ ] [ ]

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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu