Motion in Insulin [ins]

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Classification Known Fragment Motion, Shear Mechanism [F-s-2]

Structures 2HIU Conformation 2 [ PartsList ] 4INS Conformation 1 [ PartsList ]

Description Helices shear by ~1.5 Angstrom. Structures of 2 conformations have been solved but only 1 has been deposited in the Protein Data Bank. The finding that an inactive mutant of insulin had the same conformation as the crystal structure of active mutant lead some researchers (Dodson and colleagues) to suggest that the known insulin crystal structures reflect an inactive conformation, and that a conformational change is required for activity. Hua et al. presents the NMR solution structure of an active insulin mutant.

Particular values describing motion Experimental Methods = x (Traditional x-ray) Creation Date = 19970822 Modification Date = 19970822

References Q X Hua, S E Shoelson, M Kochoyan, M A Weiss (1991). Receptor binding redefined by a structural switch in a mutant human insulin. Nat. 354:238 [Medline info for 93317674] C Chothia, A M Lesk, G G Dodson and D C Hodgkin (1983). Transmission of conformational change in insulin. Nature. 302: 500-505. [Medline info for 93317674]

GO terms associated with structures Molecular function hormone activity Cellular component extracellular region Biological process physiological process

Morphs

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Best representative Morph Morph name Structure #1 Structure #2 Residues [ ] [ ]

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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu