Motion in Immunoglobulin (VL-VH movement) [igvlvh]

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Classification Known Subunit Motion, Not Involving Allostery [S-n-2]

Structures
1CBV Conf. 2 Ig BV04-01 [ PartsList ]
1GGC Conf. 1 Ig 50.1 [ PartsList ]
1GGI Conf. 2 Ig 50.1 [ PartsList ]
1NBV Conf. 1 Ig BV04-01 [ PartsList ]

Description
In immunoglobulin 50.1, upon binding peptide, VH rotates 15 degrees and translates 2.7 A relative to VL
In immunoglobulin BV04-01, upon binding DNA,VH rotates 7.5 degrees and translates 0.75 A relative to VL. There are also loop motions in the CDRs.

Particular values describing motion
Annotation Level (1..10) = 4
Maximum Rotation (degrees) = 15 (For 50.1)
Maximum Translation (A) = 2.7 (For 50.1)
Experimental Methods = x (Traditional x-ray)
Creation Date = 19970822
Modification Date = 19970822

References
S Vasudevan and D R Rose. "Conformational Flexibility in Antibodies and their Fragments," unpublished(?) manuscript. [Medline info for 93078856]
R L Stanfield, M Takimoto-Kanimura, J M Rini, A T Profy and I A Wilson (1993). Major antigen-induced domain rearragments in an antibody. Structure. 1: 83-93. [Medline info for 93078856]
J N Herron, X M He, D W Ballard, P R Blier, P E Pace, A L Bothwell, E Voss, Jr. and A B Edmundson (1991). An autoantibody to single-stranded DNA: comparison of the three- dimensional structures of the unliganded Fab and a deoxynucleotide-Fab complex. Proteins. 11: 159-175. [Medline info for 92086633]
J M Rini, R L Stanfield, E A Stura, P A Salinas, A T Profy and I A Wilson (1993). Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen. Proc Natl Acad Sci U S A. 90: 6325-6329. [Medline info for 93317674]

Data and Graphics
Graphic-1 General figure showing the immunoglobulin topology and how VH packs onto VL.

Morphs

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Best representative
Morph Morph name Structure #1 Structure #2 Residues
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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu