Motion in Immunoglobulin (VL-VH movement) [igvlvh]
[ jump to morphs ]
Classification Known Subunit Motion, Not Involving Allostery [S-n-2]
Structures 1CBV Conf. 2 Ig BV04-01 [ PartsList ] 1GGC Conf. 1 Ig 50.1 [ PartsList ] 1GGI Conf. 2 Ig 50.1 [ PartsList ] 1NBV Conf. 1 Ig BV04-01 [ PartsList ]
Description In immunoglobulin 50.1, upon binding peptide, VH rotates 15 degrees and translates 2.7 A relative to VL In immunoglobulin BV04-01, upon binding DNA,VH rotates 7.5 degrees and translates 0.75 A relative to VL. There are also loop motions in the CDRs.
Particular values describing motion Annotation Level (1..10) = 4 Maximum Rotation (degrees) = 15 (For 50.1) Maximum Translation (A) = 2.7 (For 50.1) Experimental Methods = x (Traditional x-ray) Creation Date = 19970822 Modification Date = 19970822
References S Vasudevan and D R Rose. "Conformational Flexibility in Antibodies and their Fragments," unpublished(?) manuscript. [Medline info for 93078856] R L Stanfield, M Takimoto-Kanimura, J M Rini, A T Profy and I A Wilson (1993). Major antigen-induced domain rearragments in an antibody. Structure. 1: 83-93. [Medline info for 93078856] J N Herron, X M He, D W Ballard, P R Blier, P E Pace, A L Bothwell, E Voss, Jr. and A B Edmundson (1991). An autoantibody to single-stranded DNA: comparison of the three- dimensional structures of the unliganded Fab and a deoxynucleotide-Fab complex. Proteins. 11: 159-175. [Medline info for 92086633] J M Rini, R L Stanfield, E A Stura, P A Salinas, A T Profy and I A Wilson (1993). Crystal structure of a human immunodeficiency virus type 1 neutralizing antibody, 50.1, in complex with its V3 loop peptide antigen. Proc Natl Acad Sci U S A. 90: 6325-6329. [Medline info for 93317674]
Data and Graphics Graphic-1 General figure showing the immunoglobulin topology and how VH packs onto VL.
Morphs[ show all images ]
Best representative Morph Morph name Structure #1 Structure #2 Residues IgG Fab fragment upload [ H ] upload [ L ] 244
Automatic morphs Morph Morph name Structure #1 Structure #2 Residues va1nbvH-1cbvH Fab (Bv04-01) Autoantibody Bin 1nbv [ H ] 1cbv [ H ] 219 va1cgsH-2cgrH Igg2B (Kappa) Fab Fragment Aga 1cgs [ H ] 2cgr [ H ] 214
Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu