Motion in Glycogen Phosphorylase (GP) [gp]

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Classification Known Subunit Motion, Involving Allostery [S-a-2]

Structures
1GPB Conformation 2 [ PartsList ]
9GPB Conformation 1 [ PartsList ]

Description
Quaternary Structure Changes: In the low to high affinity transition (T to R), the two subunits in a GP dimer rotate relative to each other by ~10 degrees. There are two main contact points between the subunits: the tower helices and the Cap/alpha2 contact. The subunit rotation is perpendicular to the dimer twofold and occurs about the Cap/alpha2 contact. It moves together atoms in this region by ~1 A and moves them apart near the tower helices by ~3 A. The tower helix interface completely repacks, changing the helix packing angles by ~20 degrees. Tertiary Structure Changes: the change in orientation of the tower helices causes a loop near the active site (the 280s loop) to disorder in the R form. This prevents blockage of the active site.

Particular values describing motion
Experimental Methods = x (Traditional x-ray)
Creation Date = 19970822
Modification Date = 19970822

References
D Barford and L N Johnson (1989). The allosteric transition of glycogen phosphorylase. Nature. 340: 609-616. [Medline info for 89365154]
L N Johnson and D Barford (1990). Glycogen Phosphorylase. J. Biol. Chem. 265: 2409-2412. [Medline info for 90153846]
Lin K, Rath VL, Dai SC, Fletterick RJ, Hwang PK (1996). A protein phosphorylation switch at the conserved allosteric site in GP. Science 273(5281):1539-1542 [Medline info for 96365222]

Data and Graphics
Movie and Info Page Movies and further information on GP (via UCSF)

GO terms associated with structures
Molecular functionphosphorylase activity
Biological processcarbohydrate metabolism

Morphs

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Best representative
Morph Morph name Structure #1 Structure #2 Residues
Glycogen Phosphorylase 9gpb [ ] 1gpb [ ] 842

User-submitted morphs
Morph Morph name Structure #1 Structure #2 Residues
17696-21011 Glycogen Phosphoryla upload [ A ] upload [ A ] 842
640218-14578 glycogen phosphorylase 1gpa [ A ] 1gpb [ A ] 842
615947-24142 glycogen Phosphorylase 1gpa [ A ] 1gpb [ A ] 842
648634-27177 Glycogen Phosphorylase 8gpb [ A ] 1gpa [ A ] 842


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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu