Motion in Bam HI Endonuclease [bamhi]

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Classification Known Subunit Motion, Not Involving Allostery [S-n-2]

Structures
1BAM Uncomplexed Conformation [ PartsList ]
1BHM Complex with DNA [ PartsList ]

Description
Four Changes upon DNA binding: (1) Quaternary change: 19 degree rotation of subunits in dimer moves alpha carbons up to 10 A. (2) Local ordering of 12 residues between strands 3 and 4. (3) Rearrangement of 5 residue loop after helix 6 and kinking in helix 3. (4) Unfolding of C-terminal helix into extended structure. (Most of it becomes disordered.)

Particular values describing motion
Annotation Level (1..10) = 2
Maximum CA displacement (A) = 10
Maximum Rotation (degrees) = 19 (For quaternary change)
Experimental Methods = x (Traditional x-ray)
Creation Date = 19970822
Modification Date = 19970822

References
Newman M; Strzelecka T; Dorner LF; Schildkraut I; Aggarwal AK. Structure of Bam HI endonuclease bound to DNA: partial folding and unfolding on DNA binding. Science, 1995 Aug 4, 269(5224):656-63 [Medline info for 95350655]

GO terms associated with structures
Molecular functiontype II site-specific deoxyribonuclease activity
Biological processDNA restriction-modification system

Morphs

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Best representative
Morph Morph name Structure #1 Structure #2 Residues
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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu