Motion in Aspartate Amino Transferase (AAT) [aat]

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Classification Known Domain Motion, Shear Mechanism [D-s-2]

Structures
1AMA Conformation 2 [ PartsList ]
9AAT Conformation 1 [ PartsList ]

Description
Shear motion at 2 interfaces combined with hinge in a kinked helix. The domain motion is mainly the result of just two shear motions, which occur in perpendicular directions. AAT has an active site situated between a large and a small domain, and on substrate binding the small domain closes over the active site. The major shear motion involves a 13 degree rotation of the the small domain relative to the large one. A secondary shear motion moves a helix on one side of the small domain in a direction perpendicular to both the interdomain interface and the direction of the of the other shear motion. With a 1.2 A shift and a 10 degree rotation, it drops down to cover the active site. The shear motions in AAT are facilitated by a hinge motion in a long interdomain helix. This helix is kinked by 17 degrees in the open form and changes its kink angle by 12 degrees on closure.

Particular values describing motion
Experimental Methods = x (Traditional x-ray)
Creation Date = 19970822
Modification Date = 19970822

References
E Hohenester and J N Jansonius (1994). Crystalline Mitochondrial Aspartate Aminotransferase Exists in Only Two Conformations. J. Mol. Biol. 236:963-968. [Medline info for 11340196]
C A McPhalen, M G Vincent and J N Jansonius (1992). X-ray structure refinement and comparison of 3 forms of mitochondrial aspartate-aminotransferase. J. Mol. Biol. 225: 495-517. [Medline info for 92395661]

Data and Graphics
Graphic-1 The kinking helix.

GO terms associated with structures
Molecular functiontransaminase activity, transferase activity, transferring nitrogenous groups, catalytic activity
Biological processamino acid metabolism, biosynthesis

Morphs

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Best representative
Morph Morph name Structure #1 Structure #2 Residues
9aat [ ] 1ama [ ] 401

User-submitted morphs
Morph Morph name Structure #1 Structure #2 Residues
Aspartate Aminotrans 1oxo [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase (Subgroup Ia) upload [ A ] upload [ A ] 397
Aspartate Aminotransferase (Subgroup Ib) 1bjw [ A ] 1bkg [ A ] 385
Aspartate Semialdehy upload [ B ] 1gl3 [ B ] 367

Automatic morphs
Morph Morph name Structure #1 Structure #2 Residues
Aspartate Aminotransferase 1ivr [ A ] 8aat [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 7aat [ A ] 401
Aspartate Aminotransferase 1oxo [ A ] 1akc [ A ] 401
Aspartate Aminotransferase 1oxo [ A ] 1tas [ A ] 401
Aspartate Aminotransferase 1oxp [ A ] 1tar [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 7aat [ B ] 401
Aspartate Aminotransferase 1oxo [ A ] 1ama [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 8aat [ B ] 401
Aspartate Aminotransferase 1oxo [ A ] 1ivr [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 1oxo [ B ] 401
Aspartate Aminotransferase 1oxp [ A ] 7aat [ B ] 401
Aspartate Aminotransferase 1oxo [ A ] 1akb [ A ] 401
Aspartate Aminotransferase 1oxo [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 1tar [ A ] 401
Aspartate Aminotransferase 1ivr [ A ] 1tar [ B ] 401
Aspartate Aminotransferase 1ivr [ A ] 9aat [ B ] 401
Aspartate Aminotransferase 1oxo [ B ] 1tat [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1tat [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1map [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1akb [ A ] 401
Aspartate Aminotransferase (E. 1ama [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (E. 1ama [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1akb [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1tas [ A ] 401
Aspartate Aminotransferase (E. 1akc [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (E. 1akb [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (E. 1aka [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase (E. 7aat [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1tat [ A ] 401
Aspartate Aminotransferase (E. 7aat [ B ] 1tat [ A ] 401
Aspartate Aminotransferase (E. 1ama [ A ] 7aat [ B ] 401
Aspartate Aminotransferase (E. 1akc [ A ] 9aat [ B ] 401
Aspartate Aminotransferase (E. 1akb [ A ] 9aat [ B ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1oxp [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1ama [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1ama [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1ivr [ A ] 401
Aspartate Aminotransferase (E. 1aka [ B ] 1tat [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1akc [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1maq [ A ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1tas [ A ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1akc [ A ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1tas [ B ] 401
Aspartate Aminotransferase (E. 1akc [ A ] 7aat [ B ] 401
Aspartate Aminotransferase (E. 1aka [ A ] 1ivr [ A ] 401
Aspartate Aminotransferase (E. 8aat [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase (E. 1ama [ A ] 1oxo [ B ] 401
Aspartate Aminotransferase (E. 9aat [ A ] 1oxp [ A ] 401
Aspartate Aminotransferase (E. 1akc [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (E. 1akb [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (E. 1ama [ A ] 9aat [ B ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1tat [ B ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1maq [ A ] 401
Aspartate Aminotransferase (E. 9aat [ B ] 1map [ A ] 401
Aspartate Aminotransferase (E. 8aat [ B ] 1ama [ A ] 401
Aspartate Aminotransferase (E. 8aat [ B ] 1tat [ B ] 401
Aspartate Aminotransferase (E. 1akb [ A ] 7aat [ B ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 8aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 1oxo [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 9aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 1oxo [ A ] 401
Aspartate Aminotransferase (Ma 1maq [ A ] 1oxo [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1map [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 9aat [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 1oxo [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 1tar [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 1tar [ B ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1ama [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1akc [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1tat [ B ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1map [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1akb [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1akc [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1akb [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1map [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ A ] 1tar [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1maq [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1tas [ B ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 8aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 7aat [ B ] 401
Aspartate Aminotransferase (Ma 1map [ A ] 9aat [ A ] 401
Aspartate Aminotransferase (Ma 1maq [ A ] 9aat [ A ] 401
Aspartate Aminotransferase (Ma 1map [ A ] 1oxo [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ A ] 8aat [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 8aat [ A ] 401
Aspartate Aminotransferase (Ma 1maq [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1map [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1maq [ A ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ A ] 7aat [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 1aka [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1ama [ A ] 401
Aspartate Aminotransferase (Ma 1tat [ A ] 8aat [ B ] 401
Aspartate Aminotransferase (Ma 1tat [ B ] 1tar [ B ] 401
Aspartate Aminotransferase (Ma 1tar [ A ] 1maq [ A ] 401
Aspartate Aminotransferase (Ma 1tar [ B ] 1oxp [ A ] 401
Aspartate Aminotransferase (Ma 1tas [ A ] 1tar [ B ] 401
Aspartate Aminotransferase (Ma 1tas [ B ] 1tar [ B ] 401
Aspartate Aminotransferase (Ma 1map [ A ] 7aat [ B ] 401
Aspartate Aminotransferase (Ma 1maq [ A ] 7aat [ B ] 401

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Copyright 1995-2005 M. Gerstein, W. Krebs, S. Flores, N. Echols, and others
Email: Mark.Gerstein _at_ yale.edu